Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

<p>Boster's Green Dye One Step qRT-PCR Master Mix contains all the reagents necessary for reverse transcription and PCR amplification to occur in a single PCR re-action tube, without the template. The Master Mix contains a qRT-PCR Enzyme Mix and a Green Dye qPCR MasterMix, including proprietary Reverse Transcriptase, Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo (dT)s and Random Primers. The Master Mix also has the high specificity of hot start polymerase. This Master Mix offers the user an efficient and reliable alternative to conventional “two-step” qRT-PCR. Gene-specific primers must be used along with this kit.</p>_x000D__x000D_<p><strong>More about our proprietary Reverse Transcriptase:</strong> </p>_x000D_<p>The native Reverse Transcriptase has RNase H capacity and degrades mRNA. Using a series of strategic targeted mutations, our scientists successfully nullified the RNase H activity of our RT enzyme, thus preventing RNA degradation during first-strand cDNA synthesis, resulting in higher yields and increase in the achievable length of synthesized cDNA. The engineered Reverse Transcriptase also contains a fidelity‐enhancing sub-unit which ensures superior accuracy in reverse transcription. </p>_x000D_