5x PCR-Mastermix "ready-to-load", 1000μl

5x PCR Mastermixes "ready-to-load" - super fast, very convenient and reliable.The Bio&SELL 5x PCR Mastermix "ready-to-load" is a ready-mixed solution for PCR, ready for subsequent direct application onto an agarose gel.Recommended PCR preparation:Component Volume Final concentrationBio&SELL5x PCR Mastermix 10 µl 1xPrimer 1 x µl 0.4 µMPrimer 2 x µl 0.4 µMTemplate DNA x µl 5-100 ngFill up water to 50 µl , The mix contains all necessary reagents for the PCR reaction except template DNA, primer and water. Including blue + yellow dye for direct loading onto an agarose gel.The 5x PCR Mastermix "ready-to-load" is optimised for PCR reactions with subsequent visualisation of the products by agarose gel electrophoresis. The blue dye migrates in a 1% agarose gel at the same speed as 3.5 - 4.5 kb DNA fragments, the yellow dye at about the same speed as primers (< 35 - 45 bp) . The Bio&SELL 5x PCR master mix contains Tween-20. If a detergent-free buffer system is required, please contact us at 09128 / 724 32 32.The Bio&SELL 5x PCR Mastermix is not recommended for applications with subsequent spectrophotometric measurements (absorbance or fluorescence measurements), as the two dyes may interfere with these applications.