BAFF:BAFFR [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The BAFF:BAFFR [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding between BAFF (B-cell Activating Factor) and BAFFR (BAFF receptor) . This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled BAFFR (amino acids 23-71), BAFF (amino acids 134-285), streptavidin-HRP, and assay buffer for 100 reactions.Figure 1: Illustration of the mechanism of BAFF:BAFFR [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit.A 96-well plate is coated with BAFF protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-BAFFR, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of BAFF to BAFFR.Need us to run inhibitor screens or profile your compounds against  BAFF:BAFFR? Check out ourImmunotherapy Screening Services.