HSA:FcRn Neutralizing Antibody Screening Chemiluminescence Assay Kit

The HSA:FcRn Neutralizing Antibody Screening Chemiluminescent Assay Kit is designed for the screening and profiling of neutralizing antibodies or blockers of the interaction between Human Serum Albumin (HSA) and human FcRn (Neonatal Fc receptor for IgG) . This kit comes in a convenient 96-well or 384-well format, with Biotinylated HSA and FcRn (FCGRT/B2M) (amino acids 24-297 of FCGRT and 21-119 of B2M), Streptavidin-HRP, and assay buffers for 100 or 400 reactions.Figure 1: Illustration of the mechanism behind the HSA:FcRn Neutralizing Antibody Screening Chemiluminescent Assay Kit.A 96-well or 384-well plate is coated with FcRn protein. After blocking, the plate is pre-incubated with a blocker or neutralizing antibody. Upon subsequent incubation with Biotin-HSA, the plate is treated with Streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the binding of HSA to FcRn.