PARP3 Chemiluminescent Assay Kits

The PARP3 Chemiluminescent Assay Kit is designed to measure the activity of PARP3 (poly- (ADP-ribose) polymerase 3) for screening and profiling applications. The PARP3 assay kit comes in a convenient 96-well or 384-well format, with enough recombinant purified PARP3 enzyme, reaction substrates and PARP assay buffer for 100 or 400 enzyme reactions.Figure 1. PARP3 Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP3 enzyme and an activated DNA template in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the PARP3 activity.Need us to run inhibitor screens or profile your compounds against PARP3? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.This product has beencited 4 times.