PARP2 Chemiluminescent Assay Kit

The PARP2 Chemiluminescent Assay Kit is designed to measure the activity of PARP2 (poly- (ADP-ribose) polymerase 2) for screening and profiling applications. The PARP2 assay kit comes in a convenient 96-well or 384-well format, with enough recombinant purified PARP2 enzyme (amino acids 2-583), reaction substrates, and PARP assay buffer for 100 or 400 enzyme reactions.Figure 1. PARP2 Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 384-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to PARP2 activity.This product has beencited 13 times.