MLL4 Complex Chemiluminescent Assay Kit

The MLL4 (KMT2B) Complex Chemiluminescent Assay Kit is designed to measure MLL4 (mixed lineage leukemia protein-4, also known as KMT2B) /WARD complex activity for screening and profiling applications. The MLL4/ WARD complex contains MLL4, WDR5 (WD40 repeat-containing protein 5), Ash2L (Absent, small, homeotic disks-2-like), RbBP5 (retinoblastoma-binding protein 5) and DPY30 (DumPY protein 30) . The MLL4 (KMT2B) Complex Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, primary antibody, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and enough purified MLL4/WARD complex for 100 enzyme reactions.Figure 1: Illustration of the assay principle.First, the MLL4 enzyme complex and S-adenosylmethionine are added to pre-coated with histone H3 peptide substrate plate and incubated with or without a test compound. Next, the primary antibody is added. Finally, an HRP-labeled secondary antibody is added, followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. Chemiluminescence signal is proportional to the enzymatic activity of the MLL4/WARD complex.Need us to run inhibitor screens or profile your compounds against MLL4 Complex? Check out ourMethyltransferase Screening Services.