Chemi-Verse™ PARP7 Assay Kit

The Chemi-Verse™ PARP7 Assay Kit is designed to measure the activity of PARP7 (poly- (ADP-ribose) polymerase 7) for screening and profiling applications. The PARP7 assay kit comes in a convenient 96-well or 384-well format, with enough recombinant purified PARP7 enzyme (amino acids 400-657), histone mixture, NAD+, a ribose-binding agent and PARP assay buffer for 100 or 400 enzyme reactions.Figure 1. Chemi-Verse™ PARP7 Assay Kit schematic.Histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+substrate is incubated with the PARP7 enzyme in an optimized assay buffer. The plate is then treated with an ADP-ribose specific binding agent and a secondary HRP-conjugated antibody. ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to PARP7 activity.Need us to run inhibitor screens or profile your compounds against PARP7? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.