PARP12 Chemiluminescent Assay Kit

The PARP12 Chemiluminescent Assay Kit is designed to measure the activity of PARP12 (poly- (ADP-ribose) polymerase 12) for screening and profiling applications. The PARP12 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP12 enzyme (amino acids 500-700), reaction substrates, and PARP assay buffer for 100 enzyme reactions.Figure 1. PARP12 Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP12 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the PARP12 activity.Need us to run inhibitor screens or profile your compounds against PARP12? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.