SETD2 Chemiluminescent Assay Kit

The SETD2 Direct Activity Chemiluminescent Assay Kit is designed to measure SETD2_x000D_activity for screening and profiling applications. The key to the SETD2_x000D_Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36_x000D_residue of Histone H3. With this kit, only three simple steps on a microtiter plate are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary_x000D_antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody_x000D_followed by addition of the HRP substrate to produce chemiluminescence that can then be_x000D_measured using a chemiluminescence reader.