PRMT6 (HRMT1L6) Chemiluminescent Assay Kit

The PRMT6 Direct Activity Assay kit is designed to measure PRMT6_x000D_activity for screening and profiling applications. The key to the PRMT6_x000D_Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3_x000D_residue of Histone H4. With this kit, only three simple steps on a microtiter plate are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_sample containing assay buffer and methyltransferase enzyme for one hour. Next,_x000D_primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary_x000D_antibody followed by addition of the HRP substrate to produce chemiluminescence that_x000D_can then be measured using a chemiluminescence reader.