EZH2 Chemiluminescent Assay Kit

The EZH2 Chemiluminescence Assay Kit is chemiluminescence-based assay kit designed to measure activity of the EZH2 (enhancer of zeste homolog 2) complex (EZH2/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The EZH2 Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, and enough antibody against methylated K27 residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EZH2 complex for 100 enzyme reactions.EZH2 Activity Assay Kit uses a highly specific antibody. In a first step, S-adenosylmethionine is incubated with methyltransferase and sample for one hour. This is followed by addition of the primary antibody. Finally, the plate is treated with an HRP-labeled secondary antibody and addition of the HRP substrate to produce chemiluminescence signal proportional to the EZH2 activity.This product has beencited 3 times.