FBXL10 (KDM2B) Chemiluminescent Assay Kit

The FBXL10 Chemiluminescent Assay Kit is designed to measure FBXL10_x000D_activity for screening and profiling applications. FBXL10, also known as KDM2B, JHDM1B, and_x000D_CXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyllysine_x000D_4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3. The key to the FBXL10_x000D_Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate._x000D_With this kit, only three simple steps on a microtiter plate are required for detection of_x000D_demethylase activity. First, FBXL10 enzyme is incubated with the methylated H3 peptide for one_x000D_hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary_x000D_antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be_x000D_measured using a chemiluminescence reader.