GCN5 Chemiluminescent Assay Kit

The GCN5 Chemiluminescent Assay Kit is an ELISA (enzyme-linked immunosorbent assay) -based assay designed to measure the histone acetyltransferase activity of GCN5 ((General control of amino acid synthesis, Yeast, Homolog) -like 2) for screening and profiling applications. The GCN5 assay kit comes in a convenient 96-well format, with enough recombinant purified GCN5 enzyme (amino acids 362-837), acetyl donor, pre-coated plate with histone substrate, all the reagents necessary for assay detection and blocking buffer for 96 enzyme reactions.Figure 1:Schematic representation of the mechanism behind GCN5 Chemiluminescent Assay Kit.GCN5 protein is added to a plate pre-coated with a histone substrate in the presence of acetyl-CoA. After washing, an anti-acetyl-lysine antibody is added, followed by a secondary HRP-antibody. Lastly, HRP substrate is added and the chemiluminescence signal generated can be measured. The signal is proportional to the acetyltransferase activity of GCN5Need us to run inhibitor screens or profile your compounds against GCN5? Check out ourAcetyltransferase Screening Services.​This product has beencited 1 time.