2-Step DNase I Assay Kit

The 2-Step DNase I Assay Kit is a homogeneous fluorescent based assay kit designed to measure DNase I activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant DNase I, DNA substrate, GroovyGreen™ dye, assay buffer and co-factors for 100 enzyme reactions.  The kit also contains EDTA as internal control.Figure 1: 2-Step DNase I Assay Kit mechanism.DNase I cleaves DNA molecules into nucleotides. The amount of nucleotide formed correlates directly with DNase I activity and can be quantified by the addition of a fluorescent dsDNA-binding dye which distinguishes dsDNA from free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the signal is inversely proportional to DNase I activity