PADI4 Fluorogenic Assay Kit

The PADI4 Fluorogenic Assay Kit is a fluorogenic assay designed to measure the enzymatic activity of PADI4 (protein-arginine deiminase type-4) for screening and profiling applications. This assay uses o-phthalaldehyde and DTT (dithiothreitol) as detection reagents. The assay kit comes in a convenient 96-well format, with enough purified recombinant PADI4, BAEE (Nα-Benzoyl-L-arginine ethyl ester hydrochloride), EDTA (Ethylenediaminetetraacetic acid), o-phthalaldehyde, and assay buffer for 100 reactions.Figure 1: Assay Principle.Peptidyl-arginine is converted into peptidyl-citrulline by PADI4, releasing NH4+. NH4+reacts with DTT-reduced o-phthalaldehyde. O-phthalaldehyde can then fluoresce at 470 nm, and the signal is proportional to the activity of PADI4.Need us to run inhibitor screens or profile your compounds against PADI4? Check out ourMetabolic Enzyme Screening Services.