RdRp (SARS-CoV-2) TR-FRET Assay Kit

The RdRp (SARS-CoV-2) TR-FRET Assay Kitis a TR-FRET (Time-resolved fluorescence energy transfer) designed to measure the activity of the SARS-CoV-2 RNA-dependent RNA Polymerase (RdRp) for screening and profiling applications. The RdRp (SARS-CoV-2) TR-FRET Assay Kit comes in a convenient 384-well format, with Digoxigenin-labeled RNA duplex, biotinylated ATP, RdRp assay buffer (2 components plus DTT), and purified RdRp complex.Figure 1: RdRp (SARS-CoV-2) TR-FRET Assay Kit schematic.The assay measures the direct incorporation of biotinylated ATP into the double-stranded RNA substrate. First, a test compound is incubated with the enzyme in the reaction mixture. Next, Dye-labeled Acceptor and Eu-labeled Antibody Donor are added, followed by reading of the TR-FRET signal. The increase in TR-FRET signal is proportional to the amount of ATP incorporated in the RNA.