TNKS2 Homogenous Assay Kit

The TNKS2 Homogeneous Assay Kit is designed to measure TNKS2 (tankyrase 2) activity for screening and profiling applications. The TNKS2 Homogeneous Assay Kit comes in a convenient 384-well AlphaLISA® format, with enough purified recombinant TNKS2 (amino acids 667-1166), biotinylated histone substrate, recombinant antibody (ADP-Ribose Binding Reagent 1) and assay buffer for 400 enzyme reactions.Figure 1: TNKS2 Homogenous Assay Kit schematic.A sample containing TNKS2 is incubated with a biotinylated histone substrate and NAD+for one hour. This is followed by the addition of acceptor beads and primary antibody, and finally donor beads. Alpha-counts are then measured. Alpha-counts are directly proportional to TNKS2 activity.Need us to run inhibitor screens or profile your compounds against TNKS2? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Services.