ADAM10 Fluorogenic Assay Kit

The ADAM10 Fluorogenic Assay Kit is designed to measure ADAM10 (A disintegrin and a metalloprotease 10) protease activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant ADAM10, fluorogenic substrate and solution, and assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against ADAM10? Check out ourProtease Screening Services.Figure 1: Illustration of the mechanism behind the ADAM10 Fluorogenic Assay Kit.ADAM10 is incubated with a fluorogenic substrate which is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.