PDE2A Assay Kit

The PDE2A Assay Kit is a fluorescence polarization (FP), homogeneous, 384-well assay kit designed for the screening and profiling of PDE2A (Phosphodiesterase 2A) inhibitors. This assay takes advantage of a specific fluorescent phosphate-binding nanoparticle. The kit contains enough purified recombinant PDE2A, fluorescent probe, PDE assay buffer, Binding Agent, and diluent for 400 reactions.Figure 1: Illustration of the PDE2A assay principle.The assay uses a fluorescein-labeled cyclic adenosine monophosphate (cAMP-FAM for PDE2A), in which the phosphate group is engaged within the cyclic nucleotide. This is a very small molecule that rotates fast (low FP) . PDE2A catalyzes the hydrolysis of the phosphodiester bond in the cyclic nucleotide and frees the phosphate group. In a second step the free phosphate group is recognized by a specific phosphate-binding nanobead (Binding Agent) leading to the formation of a large complex, with restricted movement (high FP) . FP is proportional to PDE2A activity.This assay requires a fluorescent microplate readercapable of measuring fluorescence polarization (FP) to read the FP signal. For more information FP technology, visit our Tech Note:FP, assay principles and applications.Need us to run inhibitor screens or profile your compounds against PDE2A? Check out ourPhosphodiesterase Screening Services.