JARID1B (KDM5B) TR-FRET Assay Kit

The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B_x000D_in a homogeneous 384 reaction format. This FRET-based_x000D_assay requires no time-consuming washing steps, making it especially suitable for high_x000D_throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient_x000D_format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue_x000D_of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and_x000D_purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly_x000D_specific antibody that recognizes demethylated substrate. With this kit, only three simple steps_x000D_on a microtiter plate are required for demethylase activity detection. First, a sample containing_x000D_JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,_x000D_dye-labeled acceptor is added followed by fluorescence reading.