JHDM1D (KDM7A) Homogeneous Assay Kit

The JHDM1D Homogeneous Assay Kit is designed to measure JHDM1D (Jumonji C domain-containing histone demethylation protein 1D) activity for screening and profiling applications. The JHDM1D Homogeneous Assay Kit comes in a convenient 96-well or 384-well AlphaLISA® format, with enough purified recombinant JHDM1D (amino acids 2-488), substrate, primary antibody, assay and detection buffer for 100 or 400 enzyme reactions.Figure 1: JHDM1D Homogenous Assay Kit schematic.A sample containing JHDM1D is incubated with a biotinylated substrate. This is followed by the addition of acceptor beads and primary antibody, and finally donor beads. Alpha-counts are then counted. Alpha-counts are proportional to JHDM1D demethylase activity.Need us to run inhibitor screens or profile your compounds against JHDM1D (KDM7A) ? Check out ourDemethylase Screening Services.