HDAC8 Fluorogenic Assay Kit

The Fluorogenic HDAC 8 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) 8 activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC activity measurements. In addition, the kit includes purified_x000D_HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative_x000D_control. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate_x000D_and developer combination. This assay method eliminates dealing with the radioactivity,_x000D_extraction, and chromatography aspects of traditional assays. Using this kit, only twoa_x000D_simple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First,_x000D_the HDAC fluorometric substrate is incubated with purified HDAC8 enzyme. The_x000D_deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence_x000D_reader.